Which phase of testing is most likely to reveal clinically significant antibodies that do not react at immediate spin or 37 C?

Which phase of testing is most likely to reveal clinically significant antibodies that do not react at immediate spin or 37 C?

• A. AHG phase
• B. Immediate spin
• C. 37°C phase
• D. Post-wash phase

Answer: (A)

The key idea is that some clinically significant antibodies are IgG that bind to red cells but do not cause visible agglutination during immediate-spin or at 37°C tests. The antiglobulin (AHG) phase uses anti-human globulin to detect these bound IgG on the cell surface, causing agglutination that would not appear in the IS or 37°C phases. So, antibodies that don’t react at immediate spin or at 37°C are most likely to be revealed during the AHG phase because AHG detects the presence of IgG coating on the cells, unmasking those clinically important antibodies.

The key idea is that some clinically significant antibodies are IgG that bind to red cells but do not cause visible agglutination during immediate-spin or at 37°C tests. The antiglobulin (AHG) phase uses anti-human globulin to detect these bound IgG on the cell surface, causing agglutination that would not appear in the IS or 37°C phases. So, antibodies that don’t react at immediate spin or at 37°C are most likely to be revealed during the AHG phase because AHG detects the presence of IgG coating on the cells, unmasking those clinically important antibodies.